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Molecular Identification of Methicillin Resistant Staphylococcus aureus Isolated from Patients with Wound Infections in Khartoum Teaching Hospital, Sudan

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Babiker O. Haroun ,Eidha A. Bin Hamee ,2011, Molecular Identification of Methicillin Resistant Staphylococcus aureus Isolated from Patients with Wound Infections in Khartoum Teaching Hospital, Sudan,Journal of Science and Technology,12 (1) ,pp:51-57

Authors:
By Babiker O. Haroun ,Eidha A. Bin Hamee ,
Year:
2011
Keywords
Staphylococcus aureus, Methicillin resistance gene, Polymerase chain reaction (PCR).
Abstract
Antimicrobial resistance has become a great public health problem worldwide and multi-drug resistant Staphylococcus aureus was widely reported. The presence or absence of methicillin resistance gene (mecA) in 48 clinical wound isolates of S. aureus was examined by the polymerase chain reaction (PCR). The results were analyzed in relation to the disk diffusion method of oxacillin (1μg). PCR was amplified at a sequence of mecA gene at 1319 bp of 9 (18.75%) isolates, and were identical to those of disk diffusion test. All strains were studied for their susceptibility to traditionally used antibiotics. The results revealed that the drug of choice for the treatment of Methicillin Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) was vancomycin and multi-drug resistance was common among MRSA strains. The study concluded that wound infections showed common multiple antibiotic resistant of MRSA and the PCR assay was found to be reliable and more accurate to detect MRSA infection.
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