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DIFFERENCES AMONG SOLANUM-SPECIES IN SODIUM CHLORIDE TOLERANCE IN VITRO

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Ayoub Zeyada Elhag ,G. Mix Wagner ,W . Horst ,2009, DIFFERENCES AMONG SOLANUM-SPECIES IN SODIUM CHLORIDE TOLERANCE IN VITRO,Journal of Science and Technology,10 (1) ,pp:1-13

Authors:
By Ayoub Zeyada Elhag ,G. Mix Wagner ,W . Horst ,
Year:
2009
Keywords
Abstract
Two-node explants of 69 potato species (58wild forms and 11cultivars) were grown for six weeks in a solid medium of Murashige and Skoog-salts (MS) containing different NaCl concentrations (0,40,80,120mM NaCl l-1. NaCl tolerance was assessed by its effects on the different growth parameters of both shoot and root (length, number and fresh weight). Large genotypic differences were apparent in NaCl tolerance, where all growth parameters were negatively affected by NaCl addition. Shoot length appeared to be among the most sensitive indicators of NaCl injury. The genotypes could be ranked according to the linear regression coefficient of relative shoot length over the three NaCl concentrations Root growth parameters were difficult to assess and some species showed poor root growth even without NaCl. So they would not be suitable as a tool for a quick screening for NaCl tolerance of potato in vitro. The shoot parameters length of the main stem of explant appeared to be more suitable for the assessment of NaCl tolerance as it is to measure and reflected clearer differences of NaCl tolerance among the species than other growth parameters. Most of the cultivars were NaCl sensitive compared to the wild forms of potato, which may provide a source of NaCl tolerance of potato.
الملخص
تمت زراعة عقل ساقية ذات برعمين ابطيين من تسعة و ستين (69) نوعاً من البطاطس (58 نوعاً برياً و11 صنفاً) ولمدة ست أسابيع في بيئة (موراشيجى واسكوج) تحتوى على تراكيز مختلفة من كلوريد الصوديوم (0؛ 40 ؛80 و120 ملمول/ل) لمدة ست أسابيع، وتم تقدير التحمل للملوحة بقياس المميزات المختلفة لنمو المجموعتين الخضري والجذري (الطول والعدد والوزن الرطب). ظهرت اختلافات كبيرة بين الأنواع في تحملها لكلوريد الصوديوم والتي انعكست على نمو العقل وكان أكثرها تأثراً طول الساق الرئيسة للعقلة، وبقياس الانحراف القياسي لها عند التراكيز المختلفة لكل نوع أمكن ترتيبها حسب تحملها لكلوريد الصوديوم. مميزات النمو الجذري صعب قياسها وبعض الأنواع أظهرت نموا جذريا ضعيفا حتى بدون كلوريد الصوديوم؛ وبالتالي تبدو غير مناسبة كأداة للفرز السريع لأنواع البطاطس بينما طول الساق الرئيسة للعقل بدا مناسبا للفرز السريع لأنواع البطاطس حسب تحملها لكلوريد الصوديوم. أصناف البطاطس المختلفة تعتبر حساسة لكلوريد الصوديوم مقارنة بالأنواع البرية والتي قد تعتبر مصدرا لتحملها في البطاطس.
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