Journals A - Z
A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z    ALL
All Volumes


How to cite

Ayoub Zeyada Elhag ,G. Mix Wagner ,W . Horst ,2009, DIFFERENCES AMONG SOLANUM-SPECIES IN SODIUM CHLORIDE TOLERANCE IN VITRO,Journal of Science and Technology,10 (1) ,pp:1-13

By Ayoub Zeyada Elhag ,G. Mix Wagner ,W . Horst ,
Two-node explants of 69 potato species (58wild forms and 11cultivars) were grown for six weeks in a solid medium of Murashige and Skoog-salts (MS) containing different NaCl concentrations (0,40,80,120mM NaCl l-1. NaCl tolerance was assessed by its effects on the different growth parameters of both shoot and root (length, number and fresh weight). Large genotypic differences were apparent in NaCl tolerance, where all growth parameters were negatively affected by NaCl addition. Shoot length appeared to be among the most sensitive indicators of NaCl injury. The genotypes could be ranked according to the linear regression coefficient of relative shoot length over the three NaCl concentrations Root growth parameters were difficult to assess and some species showed poor root growth even without NaCl. So they would not be suitable as a tool for a quick screening for NaCl tolerance of potato in vitro. The shoot parameters length of the main stem of explant appeared to be more suitable for the assessment of NaCl tolerance as it is to measure and reflected clearer differences of NaCl tolerance among the species than other growth parameters. Most of the cultivars were NaCl sensitive compared to the wild forms of potato, which may provide a source of NaCl tolerance of potato.
تمت زراعة عقل ساقية ذات برعمين ابطيين من تسعة و ستين (69) نوعاً من البطاطس (58 نوعاً برياً و11 صنفاً) ولمدة ست أسابيع في بيئة (موراشيجى واسكوج) تحتوى على تراكيز مختلفة من كلوريد الصوديوم (0؛ 40 ؛80 و120 ملمول/ل) لمدة ست أسابيع، وتم تقدير التحمل للملوحة بقياس المميزات المختلفة لنمو المجموعتين الخضري والجذري (الطول والعدد والوزن الرطب). ظهرت اختلافات كبيرة بين الأنواع في تحملها لكلوريد الصوديوم والتي انعكست على نمو العقل وكان أكثرها تأثراً طول الساق الرئيسة للعقلة، وبقياس الانحراف القياسي لها عند التراكيز المختلفة لكل نوع أمكن ترتيبها حسب تحملها لكلوريد الصوديوم. مميزات النمو الجذري صعب قياسها وبعض الأنواع أظهرت نموا جذريا ضعيفا حتى بدون كلوريد الصوديوم؛ وبالتالي تبدو غير مناسبة كأداة للفرز السريع لأنواع البطاطس بينما طول الساق الرئيسة للعقل بدا مناسبا للفرز السريع لأنواع البطاطس حسب تحملها لكلوريد الصوديوم. أصناف البطاطس المختلفة تعتبر حساسة لكلوريد الصوديوم مقارنة بالأنواع البرية والتي قد تعتبر مصدرا لتحملها في البطاطس.
  1. Vose, P. B. (1983). Rationale of selection for specific nutritional characters in crop improvement with Phaseolus vulgaris L. as a case study. In: M.R. Saric and C. Loughman (ed.), Genetic aspects of Plant Nutrition, B. Martinus Nijhoff Publishers, The Hague, Boston, Lancaster.
  2. Epstein, E. J. J. Norlyn, D.W. Rush, R. W. Kingsbur D. B. Kelley G. A. Cunning-Ham and A. F.Worna (1980). Saline culture of crops: a genetic approach, Science, 210: [399-404].
  3. Maas, E.V. (1986). Salt tolerance of plants. Appl. Agric.Res.1: [12-26].
  4. Bilski, J. J., D. C. Nelson and R.L.Conlon (1988). Response of six wild potato species to chloride and sulphate salinity, Amer. Potato J., 65: [605-612].
  5. Dix, P. J. and H. E. Street (1975). Sodium chloride-resistant cell lines from Nicotiana sylvestris and Capsicum annuum. Plant Sci. Lett. 5: [231-237].
  6. Tal, M., Heiken, H. and Dehan, K. (1978). Salt tolerance in the wild relatives of cultivated tomato: Responses of callus tissues of Lycopersicon esculentum, L.peruvianum and Solanum pennellii to high salinity, Z.P. flanzenphysiol., 86:[231-240].
  7. Elhag, A. Z., Gunda, Mix-Wagner and M. Dambroth (1994). Suitability of meristem culture for the propagation of wild and primitive forms of potato in vitro,Landbauforschung Voelkenrode, 44: [147-150].
  8. Arslan, N., G. Mix und N. El Bassam (1987). Ermittlung der Salztoleranz in vitro bei Wildarten und Kultursorten der Kartoffel.Landbauforschung Voelkenrode 37(3): [128-131].
  9. Naik, S. P. and J. M., Widholm (1993). Comparison of tissue culture and whole plants responses to salinity in potato, Plant Cell, Tissue and Organ Culture,33(3): [273-280].
  10. Khrais, Tala, Y. Leclerc and Danielle J. Donnelly (1998). Relative salinity tolerance of potato cultivars assessed by in vitro screening. Amer. J. Potato Res. 75: [207-210].
  11. Zhang, Y. and D.J. Donnelly (1997). In vitro bioassays for salinity tolerance screening of potato. Potato Res., 40: [285- 295].
  12. Ashraf, M. (1994). Breeding for salinity tolerance in plants. CRC Crit Rev. Plant Science.13: [17-42].
  13. Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant. 15: [473-497].
  14. Snedecor, G.W. and W.G. Cochran (1976). Statistical Methods, 6th ed. Iowa State University Press.
  15. Ahmad, R. and Z. N. Abdullah (1982). Potato cultivation using sea water dilution for irrigation. Z. Acker and Pflanzenbau, 151: [75-80].
  16. Levy, D., E., Fogelman and Y., Itzhag (1988). The effect of water salinity on potatoes, Solanum tuberosum L.physiological indices and yielding capacity,Potato Res. 13: [601-610].
  17. Mercado, J. A., Sancho-Carrascosa, M. A., Jimenez-Bermuezl, S., Peran-Quesada,R., Pliego-Alfaro, F. and Quesada, M. A. (2000). Assessment of in vitro growth of apical sections and adventitious organogenesis to evaluate salinity tolerance in cultivated tomato. Plant Cell, Tissue and Organ Culture 62(2): [101-106].
  18. Potluri, D. P. Sasikala and P.V. Devi Prasad (1993). Influence of salinity on auxiliary bud cultures of six lowland tropical varieties of potato, Solanum tuberosum. Plant Cell, Tissue and Organ Culture, 32: [185-191].
» Download Full Text: