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ENHANCEMENT OF GROWTH AND CONTROL OF BROWNING OF TISSUE CULTURES OF GUAVA (PSIDIUM GUAJAVA L.)

How to cite

Prof. Dr. Tagelsir Ibrahim Mohamed Idris,Elfatih M Mahd ,Abdelghaffar E. Said ,2006, ENHANCEMENT OF GROWTH AND CONTROL OF BROWNING OF TISSUE CULTURES OF GUAVA (PSIDIUM GUAJAVA L.),Journal of Science and Technology,7 (1) ,pp:0-0

Authors:
By Prof. Dr. Tagelsir Ibrahim Mohamed Idris,Elfatih M Mahd ,Abdelghaffar E. Said ,
Year:
2006
Keywords
Guava, micropropagation, browning, activated charcoal, silver nitrate.
Abstract
Difficulties reported on tissue culture of guava were mainly in the initiation phase due to the detrimental effect of phenolic browning beside the low multiplication rate and cultures decline in the proliferation phase. This study aimed to counteract browning and declining and to enhance growth and multiplication of cultures. The results revealed that better establishment of in vitro cultures of guava was obtained by vertical positioning of shoot explants and illuminated incubation. Presoaking explants in a solution containing activat-ed charcoal (AC) counteracted the adverse effect of phenolic browning on cultures vigor especially when activated charcoal (AC) was added to media of 2 passages in initiation phase. Axillary shoot formation was enhanced by a supplement of 160 mg/l adenine sulphate to multiplication media containing 1 mg/l 6-benzyladenine (BA). Upon addition of 1 mg/l silver nitrate to media, cultures decline was avoided. Rooting was enhanced by addition of 1 mg/l indol-3-butyric acid (IBA) to the rooting medium.
الملخص
أجريت هذه الدراسة بهدف التغلب على بعض الإشكالات المسببة لتدنى نجاح الزراعة النسيجية للجوافة. بناء على الدراسات السابقة صممت تجارب لمعالجة الاسمرار الفينولى في الطور الإنشائي، وتدهور المزارع وانخفاض معدلات التفرع في الطور التكاثري إضافة إلى تحفيز تجذير النبيتات. أوضحت الدراسة أفضلية الوضع الرأسي لأنسجة الجوافة في أوساط النمو مع الحضانة تحت الإضاءة الصناعية في الطور الإنشائي للزراعة في القوارير كما أن غمر الأنسجة في محلول يحوي الفحم المنشط (AC)قبل الزراعة يساعد على تجنب الآثار السالبة للاسمرار الفينولي خاصة إذا أضيف الفحم لبيئات النمو لدورتين في الطور الإنشائي. شجعت إضافة كبريتات الادنين بمعدل160 مجم/ل إلى بيئة الإكثار الحاوية على1 مجم/ل بنزايل أدنين (BA) التفرعات الجانبية وعند إضافة نترات الفضة لأوساط النمو بمعدل1 مجم/ل أمكن التغلب على تدهور المزارع النسيجية. وأمكن الحصول على أفضل تجذير عند إضافة أندول حمض البيوتريك (IBA) إلى وسط التجذير بمعدل 1مجم/ل.
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